en بیوشیمی Biochemistry Research Article Research Article <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Times New Roman;"><b>Background:</b> Mesenchymal stem cells (MSCs) are well-known for their immune-modulatory properties. A subgroup of the nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) has been recently identified to play a regulatory role in immune and inflammatory responses. This study aims to analyze and compare the gene expression levels of the NOD-like receptor family pyrin domain-containing proteins (NLRPs), such as NLRP6 and NLRP12, in Wharton&rsquo;s jelly-derived mesenchymal stem cells (WJ-MSCs) treated with interferon-gamma (IFN-&gamma;), the pro-inflammatory&nbsp;cytokine, and untreated cells.<br> <b>Methods:</b> The immunophenotypic characterization of the isolated Wharton&rsquo;s Jelly mesenchymal stem cells (WJ-MSCs) was conducted using flow cytometry. Next, they were cultured with or without IFN-&gamma;, followed by a comparison of the expression levels of the NLRP6 and NLRP12 genes using quantitative PCR (qPCR).<b><span style="font-size:9.0pt"></span></b><br> <b>Results:</b> Treatment of cells with IFN-&gamma; resulted in a statistically significant increase in NLRP12 gene expression compared to untreated cells. In contrast, the expression of NLRP6 did not differ significantly between cells with or without <span lang="EN">IFN-&gamma; treatment.</span><b><span style="font-size:9.0pt"></span></b><br> <b>Conclusion</b>: The altered expression level of NLRP12 suggests its potential role in the inflammatory regulation mediated by WJ-MSCs in response to IFN exposure; however, further studies are needed to validate its role in experimental models of inflammatory-related diseases.</span></span></div> Gene expression, Interferon-gamma, Inflammation, Mesenchymal stem cells, NOD like receptors 31 35 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1542-1&slc_lang=en&sid=1 Leila Pirdel leilapirdel@yahoo.com 100319475328460031371 100319475328460031371 Yes Department of Medicine, Ardabil Branch, Islamic Azad University, Ardabil, Iran Maryam Safajoo maryamsafajoo6900@gmail.com 100319475328460031372 100319475328460031372 No Department of Biology, Tabriz Branch, Islamic Azad University, Tabriz, Iran Masoud Maleki ma.maleki1300@gmail.com 100319475328460031373 100319475328460031373 No Department of Biology, Tabriz Branch, Islamic Azad University, Tabriz, Iran