Showing 3 results for Pangh
Mahsa Shad, Ayeneh Hagieh Pangh, Farideh Tohidi,
Volume 18, Issue 3 (May-Jun 2024)
Abstract
Background: Soil contamination with parasites poses a significant and concerning risk for the transmission of parasitic infections to humans and animals. Considering the crucial role of soil as a potential source of parasite transmission, this study aimed to investigate the presence of helminth and protozoan parasites in the soil of public parks in Gorgan City.
Methods: In this study, a total of 80 soil samples were collected from 16 public parks in Gorgan City in 2022. The soil samples were collected from various areas within the parks, including flower-making, children's play areas, walking paths, sitting areas, and garbage dumps. To isolate the parasites from the soil samples, a saturated solution of sodium nitrate was utilized. The samples were carefully examined under a microscope, and all isolates were stained and subjected to further investigation using special acid-fast stain.
Results: Toxocara eggs were the most commonly detected parasite, present in 40% of the samples (n=32). Coccidia oocysts were found in 12.5% of the samples (n=10), which could potentially be Sarcocystis or Toxoplasma oocysts. Mites were also detected in 12.5% of the samples (n=10). Additionally, Cryptosporidium oocysts were identified using Ziehl-Neelsen's modified staining method, and they were found in 10% of the samples (n=8).
Conclusion: Health education plays a vital role in raising awareness about preventive measures. Collecting reservoirs of parasites (Such as dogs and cats) and planning for the proper disposal of human waste also play an important role in prevention of this disease.
Fatemeh Livani , Faramarz Koohsar , Farideh Tohidi, Mitra Sharbatkhori , Roghiyeh Faridnia , Ganesh Yadagiri , Ayeneh Hajieh Pangh , Mehdi Khoshrou , Hamed Kalani ,
Volume 19, Issue 1 (Jan-Feb 2025)
Abstract
Background: Cutaneous leishmaniasis (CL) is a prevalent infectious zoonotic disease between humans and animals. Golestan Province is one of the important centers of CL in Iran. The current method for detecting Leishmania parasites in patients is Giemsa-stained direct smear from skin ulcers; however, PCR is a strongly recommended method for epidemiological studies. This study aimed to investigate Leishmania species in negative ulcer smears from patients suspected of having CL referred to Aq Qala Health Center using the multiplex nested PCR method.
Methods: This study was performed on 72 negative ulcer smears from patients suspected of having CL referred to Aq Qala Health Center, Golestan Province, northeastern Iran, from August 2019 to April 2020, using the multiplex nested PCR method to detect Leishmania major and Leishmania tropica species.
Results: Out of 72 samples, 4 (5.55%) samples were positive by multiplex nested PCR. Moreover, all positive samples are related to Leishmania major species.
Conclusion: The multiplex nested PCR method is strongly recommended for detecting Leishmania species in negative ulcer smears from patients suspected of having CL.
Farideh Tohidi, Abazar Nejati, Ayeneh Hagieh Pangh, Zeinolabedin Mohammadi, Mohammad Reza Ghanbari,
Volume 20, Issue 1 (1-2026)
Abstract
Background: Leishmaniasis is a zoonotic disease transmitted between humans and animals and is caused by the Leishmania parasite. This parasite is transmitted through the bite of the female sandfly. Rodents and canids serve as reservoir hosts, while humans act as incidental hosts for this parasitic disease. Given the crucial role of rodents as reservoirs for zoonotic cutaneous leishmaniasis in the endemic regions of Golestan province, we aimed to investigate the presence of Leishmania in rodents and their hard ticks in the Agh Qala and Inche Broun areas of Golestan province.
Methods: This study is a descriptive cross-sectional study. It involved the analysis of 28 liver and 28 skin samples from 28 rodents and their isolated hard ticks for the presence of Leishmania parasites using the ITS1-PCR method.
Results: In this study, 6 species were identified among the 28 rodents captured in the Agh Qala and Inche Broun areas of Golestan province, with the dominant species being Rhombomys opimus, accounting for 75% of the rodents. Through PCR analysis, 13 rodents (46.4%) and 15 hard ticks (10.7%) were positive for Leishmania major parasites. Interestingly, it was observed that 69% of the rodents infected with Leishmania parasites were female. Most rodents infected with Leishmania were found to inhabit the Inche Broun area. The majority of ticks belonged to the genera Rhipicephalus spp., Ornithonyssus bacoti, and Ixodes ricinus.
Conclusion: Given the positivity of Leishmania parasites in some ticks isolated from infected animals, it is important to consider the potential epidemiological role of hard ticks in the transmission of rodent leishmaniasis.
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