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Showing 2 results for Dastan
Detection of Aflatoxin B1 in Buffalo Feed Samples from Cities of Ahvaz and Karun
Abdol Kazem Neisi, Huria Gharibi, Afshin Takdastan, Hamideh Rezazadeh, Mina Badiee, Fatemeh Zohrehvand, Adel Nazarzadeh,
Volume 11, Issue 2 (Mar-Apr 2017)
Abstract
ABSTRACT
        Background and Objective: Mycotoxins are a group of relatively resistant toxic metabolites. The most important mycotoxins are aflatoxins (B1 and B2, G1 and G2), which originate from contaminated animal feed. Dairy cattle transmit aflatoxins B1 and B2 through milk in form of aflatoxins M1 and M2, and endanger the human health. Traditional buffalo farms play an important role in the supply of dairy products in Khuzestan Province. In addition, the province has suitable conditions for the growth of various types of fungi. Therefore, this study aimed to determine the amount of aflatoxins in buffalo feed samples collected from two main suppliers of milk in the province (Ahvaz and Karun).
         Methods: Overall, 60 samples were collected during the 3 months of autumn 2014. Samples were analyzed by the sensitive and fast method of competitive enzyme-linked immunosorbent assay.
         Results: The concentration of aflatoxin ranged from 0.77 to 64.85 μg/Kg. In addition, the concentration of aflatoxin in 21 samples was higher than the permitted limit (25 μg/Kg).
        Conclusion: The mean concentration of aflatoxin in the samples increases with the decrease in temperature and humidity. This increase is observed in Ahvaz at a higher rate, which could be due to inappropriate storage and use of rice bran in their feed.
         Keywords: Aflatoxins,  Ahvaz, Animal Feed.

Preliminary phytochemical screening and antitrichomonal activity of Ferula pseudalliacea
Zarifeh Akbari , Dara Dastan , Mohammad Fallah , Amir Hossein Maghsood , Mohammad Matini ,
Volume 19, Issue 6 (11-2025)
Abstract
Background: Trichomonas vaginalis (T. vaginalis) causes human trichomoniasis, a common type of protozoan vaginitis. Due to the increasing incidence of drug-resistant trichomoniasis, new pharmacological research is needed. The aim was to investigate the activity of Ferula pseudalliacea (F. pseudalliacea) against T. vaginalis and to perform a preliminary phytochemical analysis of its extracts.
Methods: Essential oil and various extracts of F. pseudalliacea roots, including n-hexane, ethyl acetate, and methanol, were obtained. Susceptibility testing of the plant products was performed on five T. vaginalis isolates using the microtiter plate method. Minimum lethal concentration (MLC) and growth inhibitory percent (GI%) of sub-MLC concentrations were reported after 24- and 48-hour exposures. Phytochemical screening of the extracts was carried out using a standard procedure.
Results: The antitrichomonal effect of the plant products depended on time and concentration, with the greatest effect observed after 48 hours of exposure. The essential oil and n-hexane extract of F. pseudalliacea demonstrated remarkable activity with MLC of 250 µg/ml, followed by the ethyl acetate (MLC=500 µg/ml) and methanol extract (MLC=1000 µg/ml), with GI% 92.8, 50.6, 85.2, and 42.8, respectively. The bioactive constituents of the extracts were coumarins, terpenoids, steroids, phenols, tannins, and glycosides.
Conclusion: The results of this study demonstrated in vitro antitrichomonal properties of F. pseudalliacea. Therefore, further studies are needed to investigate the potential antitrichomonal activity of its bioactive constituents.

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