Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
2538-4449
10.61186/mlj
en
jalali
1394
7
1
gregorian
2015
10
1
9
4
online
1
fulltext
fa
Using PCR to Compare the Expression of CDR1, CDR2, and MDR1 in Candida Albicans Isolates Resistant and Susceptible to Fluconazole
تحقيقي
Original Paper
<div>
<p><strong>Abstract</strong></p>
<p><strong> Background and objectives:</strong> More <em>Candida albicans</em> strains are reported resistant to fluconazole in patients with AIDS, cancer and organ recipients. Fluconazole resistance can be attributed to changes in pathways of sterol biosynthesis, mutation in or overexpression of ERG11 and the expression of CDR1, CDR2, and MDR1. This study aimed to compare the expression of CDR1, CDR2, and MDR1 in <em>C. albicans</em> resistant and susceptible to fluconazole.</p>
<p></p>
<p><strong> Methods:</strong> MIC testing for fluconazole was performed on <em>C. albicans</em> isolates isolated from patients with oral and vaginal candidiasis to determine resistant and susceptible strains. Then real time PCR was performed on the resistant and susceptible isolates and the expression of CDR1, CDR2, and MDR1 was compared in <em>C. albicans</em>.</p>
<p></p>
<p><strong> Results:</strong> Of 46 <em>Candida albicans</em> isolates, 20 susceptible isolates, 12 semi-susceptible isolates and 14 resistant isolates were identified by MIC. After real time PCR was performed, <em>Candida albicans</em> isolates susceptible to fluconazole showed moderate expression of CDR1, CDR2, and MDR1 genes, while resistant isolates showed slight or no expression.</p>
<p></p>
<p><strong> Conclusion:</strong> Increased expression of CDR1, CDR2, and MDR1 had less and insignificant role in resistance to fluconazole.</p>
<p></p>
<p><strong> Keywords:</strong> Candida Albicans, Gene Expression, Real time PCR method</p>
</div>
<p></p>
Keywords: Candida Albicans, Gene Expression, Real time PCR method
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http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-360&slc_lang=fa&sid=1
Nahid
Ariana
Ariana-nahid@yahoo.com
100319475328460011166
100319475328460011166
Yes
Faculty of Biological Sciences, Islamic Azad University, Tonekabon Branch, Iran
Ali
Nazemi
100319475328460011167
100319475328460011167
No
Islamic Azad University, Tonekabon Branch, Iran
Ayatollah
Nasrollahi Omran
100319475328460011168
100319475328460011168
No
Islamic Azad University, Tonekabon Branch, Iran