fa تحقيقي Original Paper <p>Abstract<br> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Background and Objectives: Plasmodium falciparum merozoite surface protein-1 (PfMSP-1) is a promising vaccine against malaria during its blood stages which play an important role in immunity to this disease. Polymorphic nature of this gene is a major obstacle in making an effective vaccine against malaria. In this study, the genetic diversity of Plasmodium falciparum isolates was investigated in Sistan-Baluchestan Province using allelic families of the MSP-1.<br> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Methods: From March/April 2011 to August/September 2012, 94 blood samples were collected from patients with falciparum malaria who were living in four districts of Sistan-Baluchestan Province. The extracted genomic DNA and genetic diversity of MSP-1 block 2 were evaluated by nested polymerase chain reaction.<br> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Results: From a total of 94 patients, 89 patients (94.7%) had positive PCR results and the remaining five patients were excluded. Seven different alleles of MSP-1 were identified through size difference on agarose gel. Comprising 46.1% of the samples, MAD20 was identified as the predominant MSP-1 allelic family, while the RO33 family had the lowest frequency (with 7.9%). In 10% of samples infection with two alleles was observed.<br> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Conclusion: The results of this study suggest that genetic diversity of PfMSP-1 in Southeastern Iran is relatively low and most infections originate from a clone that is consistent with an area of low malaria transmission. This information is useful for the prevention and control of malaria in Iran.<br> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Keywords: Merozoite Surface Protein 1, Plasmodium Falciparum, Polymerase Chain Reaction<br> &nbsp;</p> Merozoite Surface Protein 1, Plasmodium Falciparum, Polymerase Chain Reaction 1 5 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-355&slc_lang=fa&sid=1 Adel Ebrahimzadeh 100319475328460011153 100319475328460011153 No Research Center for Infectious and Tropical Diseases, Zahedan University of Medical Sciences, Iran Tahereh Davoodi tahereh2015@yahoo.com 100319475328460011154 100319475328460011154 Yes Zahedan University of Medical Sciences, Iran Abbas Pashaei Naghadeh 100319475328460011155 100319475328460011155 No Zabol University of Medical Sciences, Iran