en علوم آزمایشگاهی Laboratory Sciences گزارش کوتاه Brief Report <span style="font-size:11pt"><span style="line-height:200%"><span arial="" style="font-family:"><b><span style="font-size:12.0pt"><span style="background:white"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Background and objectives:</span></span></span></span></b><span style="font-size:12.0pt"><span style="background:white"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> Semen cryopreservation is widely used in assisted reproduction techniques, and reliable semen analysis is essential to define the clinical practice. However, many parameters used for semen evaluation have high variability among technicians. </span></span></span></span><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Here, we describe a method of validating semen analysis prior to cryopreservation, comparing each operator&rsquo;s results with an expert, and also analyzing inter-operator variability. As a second endpoint, we compare this method by analyzing semen parameters before and after cryopreservation.</span></span></span><b><span style="font-size:12.0pt"><span style="background:white"><span style="line-height:200%"><span style="font-family:"Times New Roman","serif""></span></span></span></span></b></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span arial="" style="font-family:"><b><span style="font-size:12.0pt"><span style="background:white"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Methods: </span></span></span></span></b><span style="font-size:12.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Four professional trainees studied and practiced semen analysis according to the World Health Organization guidelines for one month, under supervision of an expert in the field. Next, microscopic results (sperm concentration, motility, vitality, and morphology) obtained by each team member were compared with the findings obtained by the expert.. Finally, analyzes of inter-operators were evaluated for the same parameters.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span arial="" style="font-family:"><b><span style="font-size:12.0pt"><span style="background:white"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Results:</span></span></span></span></b><span style="font-size:12.0pt"><span style="background:white"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> The findings obtained by the operators and the expert did not differ significantly. Furthermore, in the inter-operator analysis, the morphology parameter differed significantly in the fresh semen sample, which was not observed in the post-thaw sample. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:200%"><span arial="" style="font-family:"><b><span style="font-size:12.0pt"><span style="background:white"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></span></b><span style="font-size:12.0pt"><span style="background:white"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""> Our results indicated that the laboratory staff training for semen analysis was effective, ensuring the assessment of individual performance and uniformity among operators in sperm count parameters, producing consistent results.</span></span></span></span></span></span></span> Semen,Semen preservation,Cryopreservation,Validation study, 47 53 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1144-1&slc_lang=en&sid=1 Karolyn Sassi Ogliari karolyn@hemocord.com.br 100319475328460022805 100319475328460022805 Yes Hemocord Biotechnology, São Leopoldo, Brazil Monica Luiza Immig monica@hemocord.com.br 100319475328460022806 100319475328460022806 No Hemocord Biotechnology, São Leopoldo, Brazil Maria Laura Halon maria.laura@hemocord.com.br 100319475328460022807 100319475328460022807 No Hemocord Biotechnology, São Leopoldo, Brazil Fabrízio Loth fabrizio@hemocord.com.br 100319475328460022808 100319475328460022808 No Hemocord Biotechnology, São Leopoldo, Brazil Luiza Boni luizaboni13@hotmail.com 100319475328460022809 100319475328460022809 No University of Vale do Rio dos Sinos, São Leopoldo, Brazil Patrícia Grudzinski patigrudzinski@gmail.com 100319475328460022810 100319475328460022810 No Hemocord Biotechnology, São Leopoldo, Brazil Liziane Beckenkamp liziane.raquel@hemocord.com.br 100319475328460022811 100319475328460022811 No Hemocord Biotechnology, São Leopoldo, Brazil Martina Fritsch martinaf@uol.com.br 100319475328460022812 100319475328460022812 No Hemocord Biotechnology, São Leopoldo, Brazil